Identification of bacterial isolates of Tumpang and Bumiasri (East Java, Indonesia) using 16S rRNA gene sequencing and screening of their active compounds as a biofertilizer

Abstract. Prihartin I, Ismail AS, Sukorini H, Nursandi F, Zakia A, Farahdina FAR. 2023. Identification of bacterial isolates Tumpang and Bumiasri (East Java, Indonesia) using 16S rRNA gene sequencing screening their active compounds as a biofertilizer. Biodiversitas 24: 3338-3343. The objective the study was to identify (TPG) (BAS) screen-on biofertilizer active-compounds. Research materials used in were TPG isolated from area BAS area, East Indonesia. variables observed isolate identification 16S-rRNA screen for liquid chromatography tandem-mass spectrometry (LC-MS/MS-QTOF). composed mineral mix (P, K, Fe, Mg S), rice-straw extract, water C1 Biofertilizer C2 Biofertilizer. results obtained DNA sequences that resembled bacterium Lysinibacillus fusiformis with similarity 99%, while macroides 99%. These genes have been submitted GenBank under names L. BIP-211 BIP-212 respectively. LC-MS/MS-QTOF result shows contain benzoic-acid compound, 4-(butylamino), methoxycinnamyl P-coumarate. Futhermore, betaine (glycine betaine) 4-(butylamino) identified C2. So, confirmed both consists two